One of these towns was located on the Napinaza River (Quebrada de Limón), Limón Indaza Canton, province of Morona Santiago. In 2004-2005, population monitoring reported a relatively stable number of individuals, although males were more abundant than females. During that research, adults and larvae showed symptoms related to the chytrid fungus (a fungus lethal to amphibians). In 2006, an investigation focused on chytrid in the same population revealed that it was present in 30% of captured individuals and two dead individuals were found (Salazar-Valenzuela 2007). In 2008, the construction of a highway severely affected part of the population due to the disposal of waste in the ravine. Since 2009, searches in the locality reported a low density of individuals and in recent years the population has disappeared from that locality. The other localities where it still survives are threatened by deforestation and mining, mainly.